Microsatellite DNA markers in Populus tremuloides.
Identifieur interne : 004789 ( Main/Exploration ); précédent : 004788; suivant : 004790Microsatellite DNA markers in Populus tremuloides.
Auteurs : M H Rahman [Canada] ; S. Dayanandan ; O P RajoraSource :
- Genome [ 0831-2796 ] ; 2000.
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Abstract
Markers for eight new microsatellite DNA or simple sequence repeat (SSR) loci were developed and characterized in trembling aspen (Populus tremuloides) from a partial genomic library. Informativeness of these microsatellite DNA markers was examined by determining polymorphisms in 38 P. tremuloides individuals. Inheritance of selected markers was tested in progenies of controlled crosses. Six characterized SSR loci were of dinucleotide repeats (two perfect and four imperfect), and one each of trinucleotide and tetranucleotide repeats. The monomorphic SSR locus (PTR15) was of a compound imperfect dinucleotide repeat. The primers of one highly polymorphic SSR locus (PTR7) amplified two loci, and alleles could not be assigned to a specific locus. At the other six polymorphic loci, 25 alleles were detected in 38 P. tremuloides individuals; the number of alleles ranged from 2 to 7, with an average of 4.2 alleles per locus, and the observed heterozygosity ranged from 0.05 to 0.61, with an average of 0.36 per locus. The two perfect dinucleotide and one trinucleotide microsatellite DNA loci were the most informative. Microsatellite DNA variants of four SSR loci characterized previously followed a single-locus Mendelian inheritance pattern, whereas those of PTR7 from the present study showed a two-locus Mendelian inheritance pattern in controlled crosses. The microsatellite DNA markers developed and reported here could be used for assisting various genetic, breeding, biotechnology, genome mapping, conservation, and sustainable forest management programs in poplars.
PubMed: 10791817
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Dayanandan</name></author><author><name sortKey="Rajora, O P" sort="Rajora, O P" uniqKey="Rajora O" first="O P" last="Rajora">O P Rajora</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:10791817</idno><idno type="pmid">10791817</idno><idno type="wicri:Area/Main/Corpus">004867</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004867</idno><idno type="wicri:Area/Main/Curation">004867</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004867</idno><idno type="wicri:Area/Main/Exploration">004867</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Microsatellite DNA markers in Populus tremuloides.</title><author><name sortKey="Rahman, M H" sort="Rahman, M H" uniqKey="Rahman M" first="M H" last="Rahman">M H Rahman</name><affiliation wicri:level="3"><nlm:affiliation>Department of Renewable Resources, Faculty of Agriculture, Forestry and Home Economics, University of Alberta, Edmonton, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Renewable Resources, Faculty of Agriculture, Forestry and Home Economics, University of Alberta, Edmonton</wicri:regionArea><placeName><settlement type="city">Edmonton</settlement><region type="state">Alberta</region></placeName></affiliation></author><author><name sortKey="Dayanandan, S" sort="Dayanandan, S" uniqKey="Dayanandan S" first="S" last="Dayanandan">S. Dayanandan</name></author><author><name sortKey="Rajora, O P" sort="Rajora, O P" uniqKey="Rajora O" first="O P" last="Rajora">O P Rajora</name></author></analytic><series><title level="j">Genome</title><idno type="ISSN">0831-2796</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alleles (MeSH)</term><term>DNA Fingerprinting (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Microsatellite Repeats (MeSH)</term><term>Minisatellite Repeats (MeSH)</term><term>Polymorphism, Genetic (MeSH)</term><term>Rosales (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Allèles (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Polymorphisme génétique (MeSH)</term><term>Profilage d'ADN (MeSH)</term><term>Rosales (génétique)</term><term>Répétitions microsatellites (MeSH)</term><term>Répétitions minisatellites (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Rosales</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Rosales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alleles</term><term>DNA Fingerprinting</term><term>Genes, Plant</term><term>Microsatellite Repeats</term><term>Minisatellite Repeats</term><term>Polymorphism, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Allèles</term><term>Gènes de plante</term><term>Polymorphisme génétique</term><term>Profilage d'ADN</term><term>Répétitions microsatellites</term><term>Répétitions minisatellites</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Markers for eight new microsatellite DNA or simple sequence repeat (SSR) loci were developed and characterized in trembling aspen (Populus tremuloides) from a partial genomic library. Informativeness of these microsatellite DNA markers was examined by determining polymorphisms in 38 P. tremuloides individuals. Inheritance of selected markers was tested in progenies of controlled crosses. Six characterized SSR loci were of dinucleotide repeats (two perfect and four imperfect), and one each of trinucleotide and tetranucleotide repeats. The monomorphic SSR locus (PTR15) was of a compound imperfect dinucleotide repeat. The primers of one highly polymorphic SSR locus (PTR7) amplified two loci, and alleles could not be assigned to a specific locus. At the other six polymorphic loci, 25 alleles were detected in 38 P. tremuloides individuals; the number of alleles ranged from 2 to 7, with an average of 4.2 alleles per locus, and the observed heterozygosity ranged from 0.05 to 0.61, with an average of 0.36 per locus. The two perfect dinucleotide and one trinucleotide microsatellite DNA loci were the most informative. Microsatellite DNA variants of four SSR loci characterized previously followed a single-locus Mendelian inheritance pattern, whereas those of PTR7 from the present study showed a two-locus Mendelian inheritance pattern in controlled crosses. The microsatellite DNA markers developed and reported here could be used for assisting various genetic, breeding, biotechnology, genome mapping, conservation, and sustainable forest management programs in poplars.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10791817</PMID><DateCompleted><Year>2000</Year><Month>06</Month><Day>26</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0831-2796</ISSN><JournalIssue CitedMedium="Print"><Volume>43</Volume><Issue>2</Issue><PubDate><Year>2000</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Genome</Title><ISOAbbreviation>Genome</ISOAbbreviation></Journal><ArticleTitle>Microsatellite DNA markers in Populus tremuloides.</ArticleTitle><Pagination><MedlinePgn>293-7</MedlinePgn></Pagination><Abstract><AbstractText>Markers for eight new microsatellite DNA or simple sequence repeat (SSR) loci were developed and characterized in trembling aspen (Populus tremuloides) from a partial genomic library. Informativeness of these microsatellite DNA markers was examined by determining polymorphisms in 38 P. tremuloides individuals. Inheritance of selected markers was tested in progenies of controlled crosses. Six characterized SSR loci were of dinucleotide repeats (two perfect and four imperfect), and one each of trinucleotide and tetranucleotide repeats. The monomorphic SSR locus (PTR15) was of a compound imperfect dinucleotide repeat. The primers of one highly polymorphic SSR locus (PTR7) amplified two loci, and alleles could not be assigned to a specific locus. At the other six polymorphic loci, 25 alleles were detected in 38 P. tremuloides individuals; the number of alleles ranged from 2 to 7, with an average of 4.2 alleles per locus, and the observed heterozygosity ranged from 0.05 to 0.61, with an average of 0.36 per locus. The two perfect dinucleotide and one trinucleotide microsatellite DNA loci were the most informative. Microsatellite DNA variants of four SSR loci characterized previously followed a single-locus Mendelian inheritance pattern, whereas those of PTR7 from the present study showed a two-locus Mendelian inheritance pattern in controlled crosses. The microsatellite DNA markers developed and reported here could be used for assisting various genetic, breeding, biotechnology, genome mapping, conservation, and sustainable forest management programs in poplars.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rahman</LastName><ForeName>M H</ForeName><Initials>MH</Initials><AffiliationInfo><Affiliation>Department of Renewable Resources, Faculty of Agriculture, Forestry and Home Economics, University of Alberta, Edmonton, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dayanandan</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Rajora</LastName><ForeName>O P</ForeName><Initials>OP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Genome</MedlineTA><NlmUniqueID>8704544</NlmUniqueID><ISSNLinking>0831-2796</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000483" MajorTopicYN="N">Alleles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016172" MajorTopicYN="N">DNA Fingerprinting</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018895" MajorTopicYN="Y">Microsatellite Repeats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018598" MajorTopicYN="Y">Minisatellite Repeats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011110" MajorTopicYN="N">Polymorphism, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019685" MajorTopicYN="N">Rosales</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>5</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>7</Month><Day>6</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>5</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10791817</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Alberta</li></region><settlement><li>Edmonton</li></settlement></list><tree><noCountry><name sortKey="Dayanandan, S" sort="Dayanandan, S" uniqKey="Dayanandan S" first="S" last="Dayanandan">S. Dayanandan</name><name sortKey="Rajora, O P" sort="Rajora, O P" uniqKey="Rajora O" first="O P" last="Rajora">O P Rajora</name></noCountry><country name="Canada"><region name="Alberta"><name sortKey="Rahman, M H" sort="Rahman, M H" uniqKey="Rahman M" first="M H" last="Rahman">M H Rahman</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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